Part:BBa_K2633001:Design

Pseudomanas sp. acdS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 701
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 478
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The gene acdS has many homologs in bacteria, and this gene in particular was easily isolated from endophytic bacteria and has a characterized structure.

Source

The amino acid sequence of acdS comes from isolated endophytic bacteria Pseudomonas sp. 1C2P-04, and primers for its amplification were based on Pseudomonas sp. UW4.